Lyme disease spirochetes induce human and murine interleukin 1 production.

摘要: IL 1 is a major immunoregulatory molecule produced by macrophages, and it appears to be the molecular orchestrator of nonspecific host defense mechanisms against variety environmental insults. Many investigators have used artificial agents stimulate macrophages produce 1. We now report production large quantities after physiologic stimulus. The Lyme disease spirochete, recently isolated adapted for growth in vitro, was P388D1 cells or human peripheral blood monocytes. Spirochetes were added confluent macrophage cultures serum-free RPMI at ratio 10:1. release dose-dependent. 24-hr supernatant activity determined using thymocyte Con A co-mitogenesis assay. Activity not due an endotoxin on, by, spirochete. polymyxin B affinity column failed remove activity, spirochete-macrophage culture had no effect on production. Supernatants collected, concentrated, subjected size exclusion HPLC. Three areas found cell supernatants (Mr greater than 60,000, 40,000, 20,000), whereas two peaks 23,000 13,000) monocyte supernatants. Mr 20,000 13,000 from murine supernatants, respectively, SDS-PAGE shown single bands 12,400 mouse 13,500 1). Isoelectric focusing column-purified preparations showed different pI both (pI 7.25 4.4 5) 5.55) Fibroblasts cultured with demonstrated increase secreted collagenase increased proliferation. Thus, stimulus simple biochemical techniques amounts very pure That this spirochete-stimulated may explain some clinical manifestations disease.