Method for the design of oligonucleotides for molecular biology techniques
作者： Andrés Octavio Aravena Duarte , Alejandro Eduardo Maass Sepúlveda , Servet Martinez Aguilera , Katia Nicole Ehrenfeld Stolzenbach , Mauricio Alejandro Gonzalez Canales
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摘要: A method of designing oligonucleotides useful in molecular biology techniques as PCR primers or other identification and/or quantification probes is disclosed. The permits specific for the a determined sequence metagenomic sample. includes selecting constructing database reference sequences, subset sequences belonging to target organisms, candidate from such depurating these according hybridization specificity and thermodynamic stability criteria, obtaining list designed that fulfill producing materially by chemical synthesis oligonucleotides, which comply with requirements desired process.
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