Bio-generation of succinic acid by fermentation of Physaria fendleri seed polysaccharides

摘要: Abstract The bio-oils research unit at the national center for agricultural utilization in Peoria, Illinois produces over 70% of crushed oilseed as press cake annually tons seed are oil. A large amount this cannot be used animal feed because anti-nutritional properties and generally winds up landfilled. Lesquerella fendleri now reclassified Physaria its belongs category processed here meal is unsuitable feed. Hence purpose study was to explore use especially polysaccharide content renewable substrate fermentative production succinic acid when crop commercialized. Success with P. would lead similar cakes. Succinic acid, an important platform chemical mainly produced industrially from maleic anhydride, a petrochemical intermediate substrate. But most based on glucose, our goal expansion sugar beyond glucose so polysaccharides crushes could become feedstuff production. Our initial trials found that ruminal microorganism strain Actinobacillus succinogenes DSM 22,257 capable producing succinate using standard sugars known components polysaccharides, i.e., arabinose, galactose, galacturonic xylose. Subsequent hydrolyzates successfully 93 >98% conversion. readily quantitatively recovered by solvent extraction heterogeneous fermentation broth. crude extract purified recrystallization >99% purity.