Tissue-based in vitro and ex vivo models for pulmonary permeability studies

摘要: Abstract The administration of drugs through the pulmonary route has gained importance in pharmaceutical field, especially for treatment several lung diseases. Lung epithelium a high surface area, with low enzymatic activity and absence first-pass metabolism. Moreover, alveolar is thin vascularized, enabling good drug permeability absorption to blood capillaries, so this option delivery local systemic effects. However, respiratory airways have anatomic features clearance mechanisms that can compromise affecting its bioavailability. Different vitro models been widely used study mechanisms, but they are still limited approaches. Isolated perfused lung, an ex vivo model, more realistic better alternative not only because structural complexity, it different factors (metabolism, mucociliary phagocytic mechanisms) influence absorption. Thus, chapter attempts provide overview main system, discusses care must be taken during harvest maintenance isolated describe use as model studying