Mixed nicotinic–muscarinic properties of the α9 nicotinic cholinergic receptor
作者： Miguel Verbitsky , Carla V Rothlin , Eleonora Katz , A Belén Elgoyhen
DOI: 10.1016/S0028-3908(00)00124-6
关键词:
摘要: Abstract The rat α9 nicotinic acetylcholine receptor (nAChR) was expressed in Xenopus laevis oocytes and tested for its sensitivity to a wide variety of cholinergic compounds. Acetylcholine (ACh), carbachol, choline methylcarbachol elicited agonist-evoked currents, giving maximal or near responses. Both the agonist suberyldicholine as well muscarinic agonists McN-A-343 methylfurtrethonium behaved weak partial receptor. Most classical compounds tested, being either (nicotine, epibatidine, cytisine, methyllycaconitine, mecamylamine, dihydro-β-erythroidine), (muscarine, atropine, gallamine, pilocarpine, bethanechol) antagonists, blocked recombinant Block by nicotine, methyllycaconitine atropine overcome at high ACh concentrations, suggesting competitive type block. present results indicate that displays mixed nicotinic–muscarinic features resemble ones described cochlear outer hair cells (OHCs). We suggest contains structural determinants responsible pharmacological properties native
elsevier.com
sci-hub.se 参考文章(58)
C Blanchet, C Erostegui, M Sugasawa, D Dulon, Acetylcholine-induced potassium current of guinea pig outer hair cells: its dependence on a calcium influx through nicotinic-like receptors The Journal of Neuroscience. ,vol. 16, pp. 2574- 2584 ,(1996) , 10.1523/JNEUROSCI.16-08-02574.1996
M.E. O'Leary, M.M. White, Mutational analysis of ligand-induced activation of the Torpedo acetylcholine receptor. Journal of Biological Chemistry. ,vol. 267, pp. 8360- 8365 ,(1992) , 10.1016/S0021-9258(18)42452-0
PA Fuchs, BW Murrow, Cholinergic inhibition of short (outer) hair cells of the chick's cochlea The Journal of Neuroscience. ,vol. 12, pp. 800- 809 ,(1992) , 10.1523/JNEUROSCI.12-03-00800.1992
A Schiavone, R Micheletti, Functional determination of McN-A-343 affinity for M1 muscarinic receptors. Journal of Pharmacology and Experimental Therapeutics. ,vol. 253, pp. 310- 314 ,(1990)
S Kakehata, T Nakagawa, T Takasaka, N Akaike, Cellular mechanism of acetylcholine‐induced response in dissociated outer hair cells of guinea‐pig cochlea. The Journal of Physiology. ,vol. 463, pp. 227- 244 ,(1993) , 10.1113/JPHYSIOL.1993.SP019592
Kazuhiko Fukuda, Tai Kubo, Isamu Akiba, Akito Maeda, Masayoshi Mishina, Shosaku Numa, Molecular distinction between muscarinic acetylcholine receptor subtypes Nature. ,vol. 327, pp. 623- 625 ,(1987) , 10.1038/327623A0
Sabine Couturier, Daniel Bertrand, Jean-Marc Matter, Maria-Clemencia Hernandez, Sonia Bertrand, Neil Millar, Soledad Valera, Thomas Barkas, Marc Ballivet, A neuronal nicotinic acetylcholine receptor subunit (α7) is developmentally regulated and forms a homo-oligomeric channel blocked by α-BTX Neuron. ,vol. 5, pp. 847- 856 ,(1990) , 10.1016/0896-6273(90)90344-F
R Boton, N Dascal, B Gillo, Y Lass, Two calcium-activated chloride conductances in Xenopus laevis oocytes permeabilized with the ionophore A23187. The Journal of Physiology. ,vol. 408, pp. 511- 534 ,(1989) , 10.1113/JPHYSIOL.1989.SP017473
Zhong-wei Zhang, Sukumar Vijayaraghavan, Darwin K. Berg, Neuronal acetylcholine receptors that bind α-bungarotoxin with high affinity function as ligand-gated ion channels Neuron. ,vol. 12, pp. 167- 177 ,(1994) , 10.1016/0896-6273(94)90161-9
T. Bonner, N. Buckley, A. Young, M. Brann, Identification of a family of muscarinic acetylcholine receptor genes Science. ,vol. 237, pp. 527- 532 ,(1987) , 10.1126/SCIENCE.3037705