IN VITRO ASSAY OF TARGET CELL LYSIS BY SENSITIZED LYMPHOCYTES

摘要: Publisher Summary Cytotoxic assay detects the presence of effector cells involved in cell-mediated immunity (CMI). By using Cr-labeled target cells, cytotoxic activity sensitized lymphocytes is detectable within less than one hour. So far vitro has been used to measure transplantation on CMI mice. Preliminary experiments indicate that same system could be soluble antigens. In this assay, lymph node peritone almacrophages and embryonic fibroblasts have also as cells. Sensitized-lymphoid are obtained after transfer from spleen, node, or thymus into lethally-irradiated allogeneic recipients.