Identification of surface proteins on bovine leukocytes by a biotin-avidin protein blotting technique

摘要: A method has been developed which covalently attaches biotin to proteins on the outer surface of leukocytes. These are separated by polyacrylamide gel electrophoresis and transferred nitrocellulose protein blotting. Labeled detected using an avidin-peroxidase conjugate color indicator. The used identify specific either polymorphonuclear leukocytes or mononuclear prepared from bovine peripheral blood. provides a highly sensitive, non-radioactive means examining alterations under differing functional physiological conditions. Cell viability is not altered this labeling labeled cells can be examine functions proteins.