Minimal residual disease after allogeneic bone marrow transplantation for chronic myeloid leukaemia in first chronic phase: correlations with acute graft-versus-host disease and relapse

摘要: We have studied 61 patients who underwent allogeneic bone marrow transplantation (BMT) for chronic myeloid leukaemia (CML) in first phase. Minimal residual disease was detected by the amplification of leukaemia-specific BCR-ABL fusion mRNA with polymerase chain reaction (PCR) using a highly sensitive nested primer strategy. As general pattern, often had detectable (PCR positive) up to 6 or 9 months post BMT after which time became undetectable negative). The conversion from PCR positive negative not associated at cyclosporin A treatment stopped. Six (10%) relapsed during period this study, two within 1 year and four more than transplant. relationship between positivity transplant relapse significant (P=0.036) but 15 were beyond remain complete clinical cytogenetic remission. Thus late identifies group increased risk is little predictive value individual patients. Of relapses, been persistently initially positive, converted subsequently again. Although all relapses preceded positivity, may occur only 12 result. proportion 3/4 found increase significantly severity acute GVHD (P=0.002) no subsequent results. There clear association