Down-regulation of long non-coding RNA TUG1 inhibits osteosarcoma cell proliferation and promotes apoptosis.
作者： Qiang Zhang , Pei-Liang Geng , Pei Yin , Xiao-Lin Wang , Jin-Peng Jia
DOI: 10.7314/APJCP.2013.14.4.2311
关键词:
摘要: Objective: To investigate the expression level of TUG1 and one its transcript variants (n377360) in osteosarcoma cells assess role proliferation apoptosis U2OS cell line. Methods: n377360 levels patients with osteosarcomas human line were evaluated using real-time quantitative PCR. transected siRNA or non-targeting siRNA. MTS was performed to flow cytometry applied analyze apoptosis. Results: We found significantly higher tissues compared matched non-tumorous tissues. In this, suppression by impaired potential cells. Furthermore, inhibition promoted Conclusions: The overexpression specimens functional regarding an provided evidence that use may be a viable but as yet unexplored therapeutic strategy tumors over express these factors.
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