Osteopontin(OPN)-induced increase in human mammary epithelial cell invasiveness is urokinase (uPA)-dependent.

摘要: We have recently shown that either exogenous or endogenous, transfected OPN induces both uPA expression and increased invasiveness of 21PT (non-tumorigenic) 21NT (tumorigenic) human mammary epithelial cells. Here we asked whether contributes functionally to the these The most invasive OPN-transfected cells were assessed for migration through Matrigel in transwell assays, presence absence various blocking antibodies inhibitors. Antibodies receptor (uPAR) significantly inhibit cell invasion, as did inhibitors (plasminogen activator inhibitor-1 [PAI-1], p-aminobenzamidine [PABN], aprotinin, amiloride). Both anti-uPA anti-uPAR inhibited invasion a level comparable control vector In contrast, non-specific IgG showed no anti-invasive effect. Cell experiments performed with parental lines is also required migratory responsiveness OPN. These data thus provide direct evidence OPN-induced requires uPA.