Human bronchial epithelial cells neoplastically transformed by v-Ki-ras: altered response to inducers of terminal squamous differentiation.

摘要: Many human bronchial adenocarcinomas have been shown to contain an activated Ki-ras oncogene (Rodenhuis et al., N. Engl. J. Med. 317 929-935, 1987). To test the hypothesis that may be causally related carcinogenesis, v-Ki-ras was transferred into established epithelial cell line, BEAS-2B, by infection with Kirsten murine sarcoma virus (Ki-MSV) or transfection a plasmid containing transforming region of Ki-MSV. These cells formed poorly differentiated in athymic nude mice. Cell lines from these tumors expressed p21 protein and were highly tumorigenic. Whereas serum growth factor beta 1 induced BEAS-2B at clonal density undergo arrest squamous differentiation, ras genes unaffected mitogenically stimulated serum.