The Effect of Methotrexate on the Folate Coenzyme Pools in Human Hepatoma Cells in Culture
作者： John Galivan , Myung Rhee , David G. Priest , Marlene Bunni , James H. Freisheim
DOI: 10.1007/978-1-4899-2638-8_16
关键词:
摘要: Recent studies on the activity of antifolates have been directed at perturbations in folate pools caused by inhibitors dihydrofolate reductase. For most part these restricted to effects methotrexate, but trimetrexate also examined. A constant feature results is elevation which accompanies inhibition both agents (1–7). variable result extent reduced coenzymes are depleted following exposure antifolates. In several cell lines reduction tetrahydrofolate quite modest (less than 40%) with 10-formyltetrahydrofolate being maintained (1–5). other cases such as L1210 cells vivo there a marked depletion coenzyme (6). Preliminary rodent hepatoma line indicated that 80% or greater and this compensated for an increase H2PteGlun 10-HCO-H2PteGlun (7). The current study extends investigations effect methotrexate human HEPG2 vitro.
springer.com
sci-hub.st 参考文章(14)
R L Seither, D F Trent, D C Mikulecky, T J Rape, I D Goldman, Folate-pool interconversions and inhibition of biosynthetic processes after exposure of L1210 leukemia cells to antifolates. Experimental and network thermodynamic analyses of the role of dihydrofolate polyglutamylates in antifolate action in cells Journal of Biological Chemistry. ,vol. 264, pp. 17016- 17023 ,(1989) , 10.1016/S0021-9258(18)71452-X
L H Matherly, C K Barlowe, V M Phillips, I D Goldman, The effects on 4-aminoantifolates on 5-formyltetrahydrofolate metabolism in L1210 cells. A biochemical basis of the selectivity of leucovorin rescue. Journal of Biological Chemistry. ,vol. 262, pp. 710- 717 ,(1987) , 10.1016/S0021-9258(19)75842-6
C J Allegra, K Hoang, G C Yeh, J C Drake, J Baram, Evidence for direct inhibition of de novo purine synthesis in human MCF-7 breast cells as a principal mode of metabolic inhibition by methotrexate. Journal of Biological Chemistry. ,vol. 262, pp. 13520- 13526 ,(1987) , 10.1016/S0021-9258(19)76458-8
P F Morrison, C J Allegra, Folate cycle kinetics in human breast cancer cells. Journal of Biological Chemistry. ,vol. 264, pp. 10552- 10566 ,(1989) , 10.1016/S0021-9258(18)81657-X
Malgorzata Balinska, John Galivan, Gladys F. Maley, Myung S. Rhee, Marlene Bunni, David G. Priest, Frank Maley, Role of Substrate Depletion in the Inhibition of Thymidylate Biosynthesis by the Dihydrofolate Reductase Inhibitor Trimetrexate in Cultured Hepatoma Cells Cancer Research. ,vol. 50, pp. 3979- 3984 ,(1990)
Marlene Bunni, D. G. Priest, F. M. Sirotnak, Relationship of reduced folate changes to inhibition of DNA synthesis induced by methotrexate in L1210 cells in vivo. Cancer Research. ,vol. 49, pp. 4204- 4209 ,(1989)
D.G. Priest, M.T. Doig, [48] Tissue folate polyglutamate chain length determination by electrophoresis as thymidylate synthase-fluorodeoxyuridylate ternary complexes☆ Methods in Enzymology. ,vol. 122, pp. 313- 319 ,(1986) , 10.1016/0076-6879(86)22186-2
David G. Priest, Marlene A. Bunni, Lorraine R. Romero-Fredes, John C. Schmitz, John M. Whiteley, A sensitive radioenzymatic assay for (S)-5-formyltetrahydrofolate. Analytical Biochemistry. ,vol. 196, pp. 284- 289 ,(1991) , 10.1016/0003-2697(91)90467-8
Ed Cadman, Christopher Benz, Robert Heimer, Joyce O'Shaughnessy, Effect of de novo purine synthesis inhibitors on 5-fluorouracil metabolism and cytotoxicity. Biochemical Pharmacology. ,vol. 30, pp. 2469- 2472 ,(1981) , 10.1016/0006-2952(81)90343-9
D.G. Priest, K.K. Happel, M. Mangum, J.M. Bednarek, M.T. Doig, C.M. Baugh, Tissue folylpolyglutamate chain-length characterization by electrophoresis as thymidylate synthetase-fluorodeoxyuridylate ternary complexes Analytical Biochemistry. ,vol. 115, pp. 163- 169 ,(1981) , 10.1016/0003-2697(81)90540-6