Chemical reporters to study mammalian O-glycosylation.

摘要: Glycans play essential roles in a range of cellular processes and have been shown to contribute various pathologies. The diversity dynamic nature glycan structures the complexities biosynthetic pathways make it challenging study specific glycans normal function disease. Chemical reporters emerged as powerful tools characterise monitor changes levels native context. A variety tags can be introduced onto monosaccharides via chemical modification endogenous or by metabolic enzymatic incorporation unnatural into glycans. These reporter strategies offer unique opportunities manipulate functions living cells whole organisms. In this review, we discuss recent advances oligosaccharide engineering chemoenzymatic labelling, focusing on their application mammalian O-linked We describe current barriers achieving labelling specificity highlight innovations that started pave way overcome these challenges.