Use of biomimetic microtissue spheroids and specific growth factor supplementation to improve tenocyte differentiation and adaptation to a collagen-based scaffold in vitro.

摘要: Tenocytes represent a valuable source of cells for the purposes tendon tissue engineering and regenerative medicine as such, should possess high degree tenogenic differentiation prior to their use in vivo order achieve maximal efficacy. In current report, we identify an efficient means by which maintain differentiated tenocytes vitro employing hanging drop technique combination with defined growth media supplements. Equine retained more state when cultured scaffold-free microtissue spheroids low serum-containing medium supplemented L-ascorbic acid 2-phosphate, insulin transforming factor (TGF)-β1. This was made evident significant increases expression levels pro-tenogenic markers collagen type I (COL1A2), III (COL3A1), scleraxis (SCX) tenomodulin (TNMD), well enhanced protein. Furthermore, under these conditions demonstrated typical spindle-like morphology embedded gels, became highly aligned respect orientation structure following migration out from spheroids. Our findings therefore provide evidence support biomimetic approach culturing that described, can improve status functional repopulation matrix.