Molekularpathologie der Lunge

摘要: Lung cancer is one of the most frequent malignancies in western world. Its association with a wide spectrum mutations genes encoding various signal transducers that are often linked to therapy response, emphasizes obvious need for improved, fast and highly efficient approaches molecular pathology. Comprehensive analyses mutation status progression relevant can be performed by novel sequencing forms named next generation (NGS) providing extremely high capacities ultra-deep sequence analyses. The 454 pyrosequencing method, synthesis semiconductor platform now available parallel multitudinous target multiple tumor DNA applications. "one molecule, clone, read" principle NGS supplies not only information on allele frequencies rates but also has advantage very sensitive detection low frequency variants.