Novel Polyoxyethylene-Containing Glycolipids Are Synthesized in Corynebacterium matruchotii and Mycobacterium smegmatis Cultured in the Presence of Tween 80

摘要: The addition of polyoxyethylene sorbitan monooleate (Tween 80) to a culture mycobacteria greatly influences cell permeability and sensitivity antibiotics but very little is known regarding the underlying mechanism. Here we show that Corynebacterium matruchotii (surrogate mycobacteria) converts Tween 80 structural series polyoxyethylenic acids which are then used form novel series-2A series-2B glycolipids. Minor series-3 glycolipids were also synthesized. replaced corynomycolic in wall. Correspondingly trehalose dicorynomycolate content was reduced. MALDI mass spectrometry, MS-MS, -NMR, -NMR characterize series-2 Series-2A glycolipid 6--corynomycolate--polyoxyethylenate 6--corynomycolate--furan ring-containing polyoxyethylenate. Mycobacterium smegmatis grown presence synthesizes type synthesis these corynebacteria should result gross changes wall drug sensitivity.