Synthetic 5'utrs, expression vectors, and methods for increasing transgene expression

作者: Thomas D. Reed

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摘要: The present invention provides synthetic 5'UTRs comprising a first polynucleotide fragment and second fragment, wherein the comprises at least one splice site of eukaryotic gene, portion 5' untranslated region is located fragment. In embodiment, intron sarcoplasmic/endoplasmic reticulum calcium ATPase gene (5'UTR) casein gene. are useful for increasing expression transgene when positioned between promoter within an vector. also vectors methods using 5'UTRs.

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