Gene Expression during Pseudotsuga Menziesii Embryogenesis: Low Molecular Weight Proteins

摘要: Somatic embryogenesis refers to the process by which embryos are initiated from a single cell or previously differentiated somatic cells. Since first reported in Picea abies (Hakman et al., 1985), has been described number of conifer species (Tautorus 1991). Progress since made development protocols for production superior quality with improved germination rates. These include modification culture medium optimizing phytohormone concentration (Gupta and Pullman, 1994) osmolarity content (Attree Fowke, 1993), desiccation treatments (Beardmore Charest, 1995; Roberts 1990). Presently, is being used as an alternative means rapid multiplication large-scale materials commercial forest 1993). The ability become embryogenic under defined condition relies on reprogramming gene expression triggers many structural changes similar those found zygotic (Misra, 1994, Tautorus As result, have provided excellent system obtain information morphological, biochemical molecular changes, take place during embryo formation early events embryogenesis. Moreover, stably transform subsequently regenerate transgenic conifers, when target transformation via particle bombardment Agrobacterium-mediated transformation, recently (Bommineni 1998; Levee 1997). use along embryogenesis-specific genes will contribute great deal genetic engineering commercially conifers elucidation regulation occurring