P450-based porous silicon biosensor for arachidonic acid detection.

摘要: A porous silicon biosensor based on P450 enzyme for arachidonic acid detection was developed. new transduction method is presented with a simultaneous measurement of refractive index and fluorescence intensity changes when the analyte binding to an surface. fluorophore bound cysteine residue in allosteric position haem domain (BMP) cytochrome BM3 enhances its upon interaction substrate acid, involved diseases such as Alzheimer's, liver cancer cellular inflammation processes. BMP has been anchored surface successfully exploited develop reaching limit 10 μM dynamic range 10-200 μM. Moreover, change also monitored at same time, displaying higher 30 Preliminary test were conducted plasma proving high specificity selectivity sensor even presence interferents 50-100 Here we suggest these two systems could be used simultaneously increase accuracy avoiding false positive response.