Embryonic Cell Extracts Ameliorate Wound Healing in Diabetic Mice

摘要: Wound healing (WH) is a physiological function in which balanced inflammatory response required for tissue regeneration and successful wound closure. In diabetic patients WH impaired negatively impacts individuals terms of morbidity, mortality social disability, while the lack an effective care highlights need novel therapeutic strategies. Extracts obtained from embryonic stem cells (EXTs) exhibited immunomodulatory properties we hypothesize that EXTs may improve diabetes. Here explored vitro vivo effect on using db/db mouse model. Our data show EXT topical administration ameliorates closure, contraction re-epithelization by significantly reducing local inflammation enhancing angiogenesis as compared to acellular protein extracts 3T3 fibroblast (3T3xt) or no treatment. increased percentage regulatory T inhibited cell activation IFNγ production vitro. Db/db-derived dendritic exposed showed decreased maturation, with downregulation costimulatory molecules CD80, CD86 CD40. On other hand, 3T3xt caused poorer closure enhanced leucocyte infiltration wounds CD40 upregulation activated both Finally, proteomic analysis revealed 82 molecular entities differentially segregating group. Following unbiased selection, 10 proteins were filtered assayed their immune activity. Apurinic/Apyrimidinic Endodeoxyribonuclease 1 (APEX1) strong at ELISpot inhibiting secretion human PBMCs. APEX1 result key factor mediating beneficial WH. upregulated tool remarkable translational potential safe, innovative cost-effective application clinic. Disclosure C. Loretelli: None. M. Ben Nasr: G. Al Haj: F. Rocchio: S. Faravelli: A. Malvandi: D9Addio: Maestroni: T. Letizia: E. Assi: V. Usuelli: Castillo Leon: R. Meroni: Zuccotti: P. Fiorina: