Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
作者： Mary Ellen Fisher , Robert Malcolm Watson
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摘要: The present invention is directed to methods for controlling the light emission of an oligonucleotide labeled with a light-emitting label that are useful in nucleic acid detection assays. A reaction results cleavage single-stranded probes carried out presence DNA binding compound interacts modify label. utilize change probe from degradation probe. applicable general assays probes, and, particular, homogeneous amplification/detection wherein hybridized cleaved concomitant primer extension. assay provided which allows simultaneous accumulation amplified target and sequence-specific sequence.
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