A DNA exonuclease induced during meiosis of Schizosaccharomyces pombe.
作者： P Szankasi , G.R. Smith
DOI: 10.1016/S0021-9258(19)50688-3
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摘要: Abstract In meiotic cells of the fission yeast Schizosaccharomyces pombe, a DNA exonuclease activity increased approximately 5-fold after premeiotic S-phase and decreased to initial level before divisions. We have purified this activity, designated I, near homogeneity. The co-purified with polypeptide an apparent molecular weight 36,000. With linear double-stranded substrate, I degraded only 5'-ended strand from each end produce 3'-single-stranded tails. enzyme also acted on nicked circular comparable affinity. induction its mode action, similar that recombination-promoting exonucleases bacteria, suggest is involved in homologous recombination S. pombe.
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