Synthesis of a monolithic, micro-immobilised enzyme reactor via click-chemistry.
作者： Bekir Çelebi , Aslıhan Bayraktar , Ali Tuncel , None
DOI: 10.1007/S00216-012-6075-7
关键词:
摘要: An immobilised enzyme reactor (IMER) in the form of capillary monolith was developed for a micro-liquid chromatography system. The plain obtained by situ thermal copolymerisation glycidyl methacrylate and ethylene dimethacrylate fused silica (200 × 0.53 mm ID) using n-propanol/1,4-butanediol as porogen. enzyme, α-chymotrypsin (CT), covalently attached onto via triazole ring formation click-chemistry. For this purpose, monolithic support treated with sodium azide reacted alkyne carrying derivative. CT linked to triazole-ring formation. activity behaviour IMER investigated system benzoyl-l-tyrosine ethyl ester (BTEE) synthetic substrate. effects mobile-phase flow rate substrate feed concentration on final BTEE conversion were under steady-state conditions. In case IMER, increased increasing concentration. Unusual explained presence convective diffusion macropores monolith. results indicated that monolithic-capillary proposed had significant advantages respect particle-based conventional high-performance liquid chromatography–IMERs.
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