EGF and bFGF pre-treatment enhances neural specification and the response to neuronal commitment of MIAMI cells

摘要: Abstract Aims Multipotent mesenchymal stromal cells raise great interest for regenerative medicine studies. Some MSC subpopulations have the potential to undergo neural differentiation, including marrow isolated adult multilineage inducible (MIAMI) cells, which differentiate into neuron-like in a multi-step neurotrophin 3-dependent manner. Epidermal and basic fibroblast growth factors are often used neuronal differentiation protocols MSCs, but with limited understanding of their role. In this study, we thoroughly assessed first time capacity these enhance MSCs. Materials methods We characterized MIAMI cell program terms stem molecule expression, cycle modifications, acquisition morphology expression molecules absence presence an EGF-bFGF pre-treatment. Results pre-treatment down-regulated stemness markers Oct4A, Notch1 Hes5, whereas neural/neuronal Nestin , Pax6 Ngn2 receptor tyrosine kinase 1 3 were up-regulated. During sustained Erk phosphorylation response NT3 was observed, began exit from exhibit increased neurite-like extensions. addition, β3-tubulin neurofilament increased; effect mediated via pathway. A slight pre-oligodendrocyte engagement noted, no default neurotransmitter phenotype observed. Overall, mesodermal unaffected or decreased, while neurogenic/adipogenic PPARγ2 incre sed. Conclusion EGF bFGF enhances specification commitment further increasing use therapy nervous system.