Subchronic Exposure of Apigenin Induces Hepatic Oxidative Stress in Male Rats

摘要: Apigenin (4’, 5, 7-trihydroxyflavone, AP), a dietary flavonoid, is reported to have several therapeutic effects in different diseases including cancer. In the present study, order explore potential mechanism and provide references for further studies, we investigated effect of apigenin at various dosages on hepatic oxidative stress male rats. Totally 48 SD rats were randomly divided into control group (saline, 1 ml/100g·bw), low-dose (AP, 234 mg/kg·bw), middle-dose 468 mg/kg·bw) high-dose 936 mg/kg·bw). The administered with or saline via intragastriation once day, 6 days per week, 5 consecutive weeks. Rats sacrificed livers harvested then immediately preserved ﹣20°C. Liver homogenate was prepared before detection. Hepatic malondialdehyde (MDA), nitric oxide syntheses (NOS), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC) (GSH) determined by colorimetric methods according provided procedures. weights liver spleen treatment groups did not reveal statistically significant difference when compared that (P > 0.05). Total protein (TP), albumin (ALB) globulin (GLO) significantly lower than those < SOD higher T-AOC, CAT GSH-Px AP can result reduction thus leading damage tissues. contrast, apegenin reduce elimination oxygen free radicals.