Following a protein kinase activity using a field-effect transistor device

作者: Ronit Freeman , Ron Gill , Itamar Willner

DOI: 10.1039/B707677K

关键词:

摘要: The specific phosphorylation of a peptide-functionalized ion-sensitive field-effect transistor device by casein kinase II in the presence ATP enables electronic readout protein activity; treatment phosphorylated surface with alkaline phosphatase results regeneration active sensing surface.

参考文章(17)
Olga?A. Biloivan, Sergiy?V. Dzyadevych, Olga?A. Boubriak, Alexey?P. Soldatkin, Anna?V. El'skaya, Development of Enzyme Biosensor Based on ISFETs for Quantitative Analysis of Serine Proteinases Electroanalysis. ,vol. 16, pp. 1883- 1889 ,(2004) , 10.1002/ELAN.200403076
Huamin Wang, Alan Davis, Shihui Yu, Khalil Ahmed, Response of cancer cells to molecular interruption of the CK2 signal. Molecular and Cellular Biochemistry. ,vol. 227, pp. 167- 174 ,(2001) , 10.1023/A:1013112908734
Maya Zayats, Andrei B Kharitonov, Eugenii Katz, Andreas F Bückmann, Itamar Willner, An integrated NAD+-dependent enzyme-functionalized field-effect transistor (ENFET) system: development of a lactate biosensor. Biosensors and Bioelectronics. ,vol. 15, pp. 671- 680 ,(2000) , 10.1016/S0956-5663(00)00120-2
F. Uslu, S. Ingebrandt, D. Mayer, S. Böcker-Meffert, M. Odenthal, A. Offenhäusser, Labelfree fully electronic nucleic acid detection system based on a field-effect transistor device Biosensors and Bioelectronics. ,vol. 19, pp. 1723- 1731 ,(2004) , 10.1016/J.BIOS.2004.01.019
Sergei V. Dzyadevych, Alexey P. Soldatkin, Anna V. El'skaya, Claude Martelet, Nicole Jaffrezic-Renault, Enzyme biosensors based on ion-selective field-effect transistors. Analytica Chimica Acta. ,vol. 568, pp. 248- 258 ,(2006) , 10.1016/J.ACA.2005.11.057
Benjamin T. Houseman, Joon H. Huh, Stephen J. Kron, Milan Mrksich, Peptide chips for the quantitative evaluation of protein kinase activity. Nature Biotechnology. ,vol. 20, pp. 270- 274 ,(2002) , 10.1038/NBT0302-270
J. Fritz, E. B. Cooper, S. Gaudet, P. K. Sorger, S. R. Manalis, Electronic detection of DNA by its intrinsic molecular charge Proceedings of the National Academy of Sciences of the United States of America. ,vol. 99, pp. 14142- 14146 ,(2002) , 10.1073/PNAS.232276699
Jang-Kyoo Shin, Dong-Sun Kim, Hey-Jung Park, Geunbae Lim, Detection of DNA and Protein Molecules Using an FET‐Type Biosensor with Gold as a Gate Metal Electroanalysis. ,vol. 16, pp. 1912- 1918 ,(2004) , 10.1002/ELAN.200403080
Maya Zayats, Ye Huang, Ron Gill, Chun-an Ma, Itamar Willner, Label-Free and Reagentless Aptamer-Based Sensors for Small Molecules Journal of the American Chemical Society. ,vol. 128, pp. 13666- 13667 ,(2006) , 10.1021/JA0651456
Svetlana P. Pogorelova, Maya Zayats, Andrei B. Kharitonov, Eugenii Katz, Itamar Willner, Analysis of NAD(P)+-cofactors by redox-functionalized ISFET devices Sensors and Actuators B-chemical. ,vol. 89, pp. 40- 47 ,(2003) , 10.1016/S0925-4005(02)00425-2