LncRNA FEZF1-AS1 enhances epithelial-mesenchymal transition (EMT) through suppressing E-cadherin and regulating WNT pathway in non-small cell lung cancer (NSCLC).
作者： Rong He , Fei hu Zhang , Ning Shen
DOI: 10.1016/J.BIOPHA.2017.08.057
关键词:
摘要: Abstract Objective Recent discoveries verify that long non-coding RNAs (lncRNAs) are important functional regulators involved in non-small cell lung cancer (NSCLC) progression. However, RNA FEZF1-AS1 was not been investigated NSCLC so for. Methods We applied the quantitative real time polymerase chain reaction (qRT-PCR) assays to detect expression of lncRNA tissues and adjacent normal tissues. Cell proliferation invasion capacities were evaluated by MTT, colony formation, assays. Chromatin immunoprecipitation (ChIP) (RIP) methods demonstrated association between E-cadherin. The relative protein levels analyzed western blot analysis. Results LncRNA significantly up-regulated compared with Higher associated lymph node metastasis, poor differentiation grade advanced TNM stage. In vitro, we revealed down-regulation inhibited NSCLC. Moreover, suppressed epithelial-mesenchymal transition (EMT) process increasing E-cadherin ZO-1, whereas, decreasing Slug, Twist Vimentin cells. Furthermore, could epigenetically repress via binding LSD1 EZH2 also knockdown Wnt/β-catenin signaling Conclusion These results function as a tumor promoting regulator NSCLC, which may provide target treatment
em-consulte.com参考文章(22)
Ningning Cheng, Weijing Cai, Shengxiang Ren, Xuefei Li, Qi Wang, Hui Pan, Mingchuan Zhao, Jiayu Li, Yishi Zhang, Chao Zhao, Xiaoxia Chen, Ke Fei, Caicun Zhou, Fred R. Hirsch, Long non-coding RNA UCA1 induces non-T790M acquired resistance to EGFR-TKIs by activating the AKT/mTOR pathway in EGFR -mutant non-small cell lung cancer Oncotarget. ,vol. 6, pp. 23582- 23593 ,(2015) , 10.18632/ONCOTARGET.4361
Michael B. Clark, John S. Mattick, Long noncoding RNAs in cell biology Seminars in Cell & Developmental Biology. ,vol. 22, pp. 366- 376 ,(2011) , 10.1016/J.SEMCDB.2011.01.001
Charles S. Dela Cruz, Lynn T. Tanoue, Richard A. Matthay, Lung Cancer: Epidemiology, Etiology, and Prevention Clinics in Chest Medicine. ,vol. 32, pp. 605- 644 ,(2011) , 10.1016/J.CCM.2011.09.001
John S Mattick, John L Rinn, Discovery and annotation of long noncoding RNAs Nature Structural & Molecular Biology. ,vol. 22, pp. 5- 7 ,(2015) , 10.1038/NSMB.2942
Rebecca Siegel, Carol DeSantis, Katherine Virgo, Kevin Stein, Angela Mariotto, Tenbroeck Smith, Dexter Cooper, Ted Gansler, Catherine Lerro, Stacey Fedewa, Chunchieh Lin, Corinne Leach, Rachel Spillers Cannady, Hyunsoon Cho, Steve Scoppa, Mark Hachey, Rebecca Kirch, Ahmedin Jemal, Elizabeth Ward, Cancer treatment and survivorship statistics, 2012 CA: A Cancer Journal for Clinicians. ,vol. 62, pp. 220- 241 ,(2012) , 10.3322/CAAC.21149
Na Chen, Dan Guo, Qiong Xu, Minhui Yang, Dan Wang, Man Peng, Yanqing Ding, Shuang Wang, Jun Zhou, Long non-coding RNA FEZF1-AS1 facilitates cell proliferation and migration in colorectal carcinoma Oncotarget. ,vol. 7, pp. 11271- 11283 ,(2016) , 10.18632/ONCOTARGET.7168
Samriddhi Shukla, Sonam Sinha, Sajid Khan, Sudhir Kumar, Kavita Singh, Kalyan Mitra, Rakesh Maurya, Syed Musthapa Meeran, Cucurbitacin B inhibits the stemness and metastatic abilities of NSCLC via downregulation of canonical Wnt/β-catenin signaling axis. Scientific Reports. ,vol. 6, pp. 21860- 21860 ,(2016) , 10.1038/SREP21860
Chongshuang Zang, Feng-qi Nie, Qian Wang, Ming Sun, Wei Li, Jing He, Meiling Zhang, Kai-hua Lu, Long non-coding RNA LINC01133 represses KLF2, P21 and E-cadherin transcription through binding with EZH2, LSD1 in non small cell lung cancer Oncotarget. ,vol. 7, pp. 11696- 11707 ,(2016) , 10.18632/ONCOTARGET.7077
W Li, M Sun, C Zang, P Ma, J He, M Zhang, Z Huang, Y Ding, Y Shu, Upregulated long non-coding RNA AGAP2-AS1 represses LATS2 and KLF2 expression through interacting with EZH2 and LSD1 in non-small-cell lung cancer cells Cell Death and Disease. ,vol. 7, pp. 0- ,(2016) , 10.1038/CDDIS.2016.126
Yousheng Mao, Ding Yang, Jie He, Mark J. Krasna, Epidemiology of Lung Cancer Surgical Oncology Clinics of North America. ,vol. 25, pp. 439- 445 ,(2016) , 10.1016/J.SOC.2016.02.001