Molecular Characterization of Pathogenic Members of the Genus Fonsecaea Using Multilocus Analysis

摘要: Members of the fungal genus Fonsecaea causing human chromoblastomycosis show substantial geographic structuring. Genetic identity clinical and environmental strains suggests transmission from plant debris, while evolutionary processes that have led to spatially separated populations remained unexplained. Sequences ITS, BT2, ACT1, Cdc42, Lac HmgA were analyzed, either by direct sequencing or cloning. Thirty-seven Central South America, Asia, Africa Europe sequenced possible recombination events calculated. Phylogenetic trees statistically supported, but BT2 ACT1 not. The Standardized Index Association (I(A) (S)) did not detect (S) = 0.4778), neither Phi-test for separate genes. In nubica non-synonymous mutations functional changes observed in gene, even though no selection pressures detected with neutrality test (Tajima D test, p>0.05). differentiation each gene showed separation American, African Asian populations. Strains vs. origin genetic distances comparable lower than found differentiation. conclusion, here we demonstrated clonality sibling species using multilocus data, structuring populations, a low structural selective constraint during evolution Fonsecaea.