[31] Crystalline propionyl-CoA carboxylase from pig heart

摘要: Publisher Summary The chapter describes two methods for the assay of propionyl-CoA carboxylase. In one, rate fixation bicarbonate- 14 C to is followed by measuring increase in acid-insoluble radioactivity. other, formation ADP measured spectrophotometrically coupling carboxylase reaction with reactions catalyzed pyruvate kinase and lactate dehydrogenase. With an excess dehydrogenase, oxidation NADH proportional ADP, therefore HCO -3 yield Ds -methylmalonyl-CoA. At early stages purification, presence ATPase necessitates use H CO assay. nucleoside 5’-triphosphate specificity pig heart restricted ATP. Regarding its fatty acyl-CoA specificity, enzyme active, although a much lesser extent, butyryl-, acetyl-, crotonyl-CoA besides propionyl-CoA. Propionyl-CoA also purified from acetone powder bovine liver mitochondria, found Rhodospirillum rubrum, Mycobacterium smegmatis.