New Motilities and Motors in the Flagella of Chlamydomonas

摘要: Chlamydomonas is a haploid, unicellular green alga, about 10 µm in diameter. Synchronous cultures can be grown phototrophically simple defined medium. In addition to the two 10–12 µm-long flagella that emanate from one end of cell, shares cytology typical higher plant and animal cells (e.g., basal bodies/centrioles, microtubules, actin filaments, chloroplasts, mitochondria, en-doplasmic reticulum, Golgi). The genetics similar yeast, with plus minus mating form zygotes, followed by meiosis tetrad formation. Many mutants are available (Harris 1989) or generated classical mutagenesis procedures. Because easily transformed, introduced DNA inserting randomly into genome (Diener et al. 1990; Kindle 1989), also insertional mutagenesis, allowing for isolation mutant genes plasmid rescue (Tam Lefebvre 1993). addition, progress being made on gene cloning complementation (Purton Rochaix 1994) selection homologous recombinants (Sodeinde We using flagellar regeneration this alga as model system studying biogenesis cell organelles (Johnson Rosenbaum