作者: S. WANARATANA , S. PANYIM , S. PAKPINYO
DOI: 10.1111/J.1365-2915.2010.00928.X
关键词:
摘要: The objective of the present study was to determine potential for house flies (Musca domestica L.) (Diptera: Muscidae) harbour avian influenza (AI) H5N1 virus. Laboratory-reared were experimentally fed with a mixture containing AI Exposed washed brain-heart infusion broth and followed by 70% alcohol before preparation whole fly homogenate. homogenate inoculated into six 10-day-old embryonated chicken eggs (ECEs). Allantoic fluids collected virus using haemagglutination (HA) test, reverse transcription-polymerase chain reaction (RT-PCR) or quantitative real-time RT-PCR (RRT-PCR). In first experiment, ECEs that 50 exposed homogenates died within 48 h HA positive second only one test RT-PCR. last group at 0, 6, 12, 24, 36, 48, 72 96 post-exposure. Fly each time point tested titration in RRT-PCR. Virus titres declined relation exposure time. Furthermore, RRT-PCR results any point. shows may could act as mechanical vector