The potential of house flies to act as a vector of avian influenza subtype H5N1 under experimental conditions

作者: S. WANARATANA , S. PANYIM , S. PAKPINYO

DOI: 10.1111/J.1365-2915.2010.00928.X

关键词:

摘要: The objective of the present study was to determine potential for house flies (Musca domestica L.) (Diptera: Muscidae) harbour avian influenza (AI) H5N1 virus. Laboratory-reared were experimentally fed with a mixture containing AI Exposed washed brain-heart infusion broth and followed by 70% alcohol before preparation whole fly homogenate. homogenate inoculated into six 10-day-old embryonated chicken eggs (ECEs). Allantoic fluids collected virus using haemagglutination (HA) test, reverse transcription-polymerase chain reaction (RT-PCR) or quantitative real-time RT-PCR (RRT-PCR). In first experiment, ECEs that 50 exposed homogenates died within 48 h HA positive second only one test RT-PCR. last group at 0, 6, 12, 24, 36, 48, 72 96 post-exposure. Fly each time point tested titration in RRT-PCR. Virus titres declined relation exposure time. Furthermore, RRT-PCR results any point. shows may could act as mechanical vector

参考文章(26)
Carlos Pijoan, Satoshi Otake, Roger D. Moon, Scott A. Dee, MacDonald Farnham, Kurt D. Rossow, Carlos Trincado, Survival of porcine reproductive and respiratory syndrome virus in houseflies Canadian Journal of Veterinary Research-revue Canadienne De Recherche Veterinaire. ,vol. 67, pp. 198- 203 ,(2003)
Kamlang Chumpolbanchorn, Kridsada Chaichoune, Bopit Puyati, Nakrop Siripara, Namphung Suemanotham, The effect of temperature and UV light on infectivity of avian influenza virus (H5N1, Thai field strain) in chicken fecal manure. Southeast Asian Journal of Tropical Medicine and Public Health. ,vol. 37, pp. 102- 105 ,(2006)
S. W. Tan, K. L. Yap, H. L. Lee, Mechanical transport of rotavirus by the legs and wings of Musca domestica (Diptera: Muscidae). Journal of Medical Entomology. ,vol. 34, pp. 527- 531 ,(1997) , 10.1093/JMEDENT/34.5.527
Bernard Greenberg, FLIES AND DISEASE. Scientific American. ,vol. 213, pp. 92- 99 ,(1965) , 10.1038/SCIENTIFICAMERICAN0765-92
Antonio J De Jesús, Alan R Olsen, John R Bryce, Richard C Whiting, Quantitative contamination and transfer of Escherichia coli from foods by houseflies, Musca domestica L. (Diptera: Muscidae) International Journal of Food Microbiology. ,vol. 93, pp. 259- 262 ,(2004) , 10.1016/J.IJFOODMICRO.2003.12.003
Maike Förster, Sven Klimpel, Heinz Mehlhorn, Kai Sievert, Sabine Messler, Klaus Pfeffer, Pilot study on synanthropic flies (e.g. Musca, Sarcophaga, Calliphora, Fannia, Lucilia, Stomoxys) as vectors of pathogenic microorganisms. Parasitology Research. ,vol. 101, pp. 243- 246 ,(2007) , 10.1007/S00436-007-0522-Y
C. CAFARCHIA, R. P. LIA, D. ROMITO, D. OTRANTO, Competence of the housefly, Musca domestica, as a vector of Microsporum canis under experimental conditions. Medical and Veterinary Entomology. ,vol. 23, pp. 21- 25 ,(2009) , 10.1111/J.1365-2915.2008.00785.X
H. Chen, G. Deng, Z. Li, G. Tian, Y. Li, P. Jiao, L. Zhang, Z. Liu, R. G. Webster, K. Yu, The evolution of H5N1 influenza viruses in ducks in southern China Proceedings of the National Academy of Sciences of the United States of America. ,vol. 101, pp. 10452- 10457 ,(2004) , 10.1073/PNAS.0403212101
Sunchai Payungporn, Salin Chutinimitkul, Arunee Chaisingh, Sudarat Damrongwantanapokin, Chantanee Buranathai, Alongkorn Amonsin, Apiradee Theamboonlers, Yong Poovorawan, Single step multiplex real-time RT-PCR for H5N1 influenza A virus detection. Journal of Virological Methods. ,vol. 131, pp. 143- 147 ,(2006) , 10.1016/J.JVIROMET.2005.08.004
D. Nayduch, G. Pittman Noblet, F. J. Stutzenberger, Vector potential of houseflies for the bacterium Aeromonas caviae. Medical and Veterinary Entomology. ,vol. 16, pp. 193- 198 ,(2002) , 10.1046/J.1365-2915.2002.00363.X