Further studies on the induction of alkaline phosphatase by 5-bromodeoxyuridine in a hybrid line between mouse and Chinese hamster in culture

摘要: Abstract The thymidine analog 5-bromodeoxyuridine induced the alkaline phosphatase activity in a hybrid line (B-6), clone of mouse × Chinese hamster cell hybrids vitro . 5-Bromodeoxyuridine at 5 μg/ml or less had minor effect on ability cells to grow and survive even during relatively prolonged subcultivation (18 days). In contrast, was increased following lag time 24 h after exposure reached its maximum period from 6–9 days treatment. This level returned original removal further incubation normal medium. Thymidine suppressed induction when given together with cultures. also observed either 5-iododeoxyuridine 5-bromodeoxycytidine. has been already reported by us inhibit hyaluronic acid synthesis used here. comparing these two, rather inverse effects fo cells, there found an intimate correlation between them. It is therefore suggested that suppression are caused same common mechanisms treated analog.