Effects of acylation on the structure, lipid binding, and transfer activity of wheat lipid transfer protein.
作者： Christine Pato , Vinh Tran , Didier Marion , Jean Paul Douliez , None
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摘要: Study of the effect protein chemical acylation on their functional properties or activity often brings valuable information regarding structure-function relationships. We performed such work wheat lipid transfer protein, LTP1, to investigate role grafted acyl chains binding and properties. LTP1 was acylated by using anhydride derivatives various chain lengths from C2 C6. Only modifications with hexanoic acid yielded a marked tertiary structure slight change in secondary structure. The affinity modified proteins for myristoyl-lysophosphatidylcholine similar that native accompanied decrease stoichiometry. Interestingly, enhanced at least factor 10 length. Finally, grafting investigated means molecular modelling, an attempt is made correlate our experimental data.
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A. Gerbanowski, C. Rabiller, C. Larré, J. Guéguen, Grafting of aliphatic and aromatic probes on bovine serum albumin: influence on its structural and physicochemical characteristics. Journal of Protein Chemistry. ,vol. 18, pp. 325- 336 ,(1999) , 10.1023/A:1021043529923
Edith Gincel, Jean-Pierre Simorre, Anita Caille, Didier Marion, Marius Ptak, Francoise Vovelle, Three-dimensional structure in solution of a wheat lipid-transfer protein from multidimensional 1H-NMR data. A new folding for lipid carriers. FEBS Journal. ,vol. 226, pp. 413- 422 ,(1994) , 10.1111/J.1432-1033.1994.TB20066.X
Jean-Paul Douliez, Christine Pato, Hanitra Rabesona, Daniel Mollé, Didier Marion, Disulfide bond assignment, lipid transfer activity and secondary structure of a 7-kDa plant lipid transfer protein, LTP2. FEBS Journal. ,vol. 268, pp. 1400- 1403 ,(2001) , 10.1046/J.1432-1327.2001.02007.X
Eric Record, Laurence Lesage, Bernard Cahagnier, Didier Marion, Marcel Asther, Filamentous fungi with high cytosolic phospholipid transfer activity in the presence of exogenous phospholipid. Applied and Environmental Microbiology. ,vol. 60, pp. 3390- 3395 ,(1994) , 10.1128/AEM.60.9.3390-3395.1994
Christine Pato, Marc Le Borgne, Guillaume Le Baut, Patrice Le Pape, Didier Marion, Jean-Paul Douliez, Potential application of plant lipid transfer proteins for drug delivery. Biochemical Pharmacology. ,vol. 62, pp. 555- 560 ,(2001) , 10.1016/S0006-2952(01)00708-0
Kathleen J. Barber, Joseph J. Warthesen, Some functional properties of acylated wheat gluten Journal of Agricultural and Food Chemistry. ,vol. 30, pp. 930- 934 ,(1982) , 10.1021/JF00113A030
Jean-Paul Douliez, Thierry Michon, Didier Marion, Steady-state tyrosine fluorescence to study the lipid-binding properties of a wheat non-specific lipid-transfer protein (nsLTP1) Biochimica et Biophysica Acta. ,vol. 1467, pp. 65- 72 ,(2000) , 10.1016/S0005-2736(00)00197-8
A. Gerbanowski, C. Malabat, C. Rabiller, J. Guéguen, Grafting of aliphatic and aromatic probes on rapeseed 2S and 12S proteins: influence on their structural and physicochemical properties. Journal of Agricultural and Food Chemistry. ,vol. 47, pp. 5218- 5226 ,(1999) , 10.1021/JF990226P
Stephen J. Chambers, Helen J. Carr, Nigel Lambert, An investigation of the dissociation and denaturation of legumin by salts using laser light scattering and circular dichroism spectroscopy Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. ,vol. 1037, pp. 66- 72 ,(1990) , 10.1016/0167-4838(90)90102-L
Alain Zachowski, Francoise Guerbette, Michele Grosbois, Alain Jolliot-Croquin, Jean-Claude Kader, Characterisation of acyl binding by a plant lipid-transfer protein. FEBS Journal. ,vol. 257, pp. 443- 448 ,(1998) , 10.1046/J.1432-1327.1998.2570443.X