Construction of a dense genetic linkage map for apple rootstocks using SSRs developed from Malus ESTs and Pyrus genomic sequences

摘要: Marker-assisted selection (MAS) offers quick and reliable prediction of the phenotypes seedlings in large populations thus opens new approaches for to breeders apple (Malus x domestica Borkh.). The development framework maps enables discovery genetic markers linked desired traits. Although have been reported scion cultivars, none has previously constructed rootstocks. We report construction a cross between ‘M.9’ (‘Malling 9’) ‘R.5’ (‘Robusta 5’) comprise 224 simple sequence repeat (SSR) markers, 18 sequence-characterised amplified regions, 14 single nucleotide polymorphisms 42 random polymorphic DNAs. A set 47 SSRs was developed from EST sequences used this rootstock map. All 17 linkage groups identified aligned existing maps. span 1,175.7 cM (‘M.9’) 1,086.7 (‘R.5’). To improve efficiency mapping map, we bin set. Applications these include elucidation basis dwarfing effect analysis disease insect resistance traits such as fire blight (Erwinia amylovora), scab (Venturia inaequalis) woolly aphid (Eriosoma lanigerum). Markers mapped population will be direct use MAS identification causative genes by map-based cloning.