Surface-enhanced Raman spectroscopic investigation of bacterial lipolysis in a skin pore phantom
作者： Millicent K. Weldon , Michael D. Morris
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摘要: Bacterial lipolysis of triglycerides is followed in a sebum pore phantom by microprobe surface-enhanced Raman scattering (SERS) spectroscopy. The consists purpose-built syringe pump operating at physiological flow rates coupled to 300 mu m i.d. capillary. We employ silicon substrate SERS microprobes (50 X 700 m) monitor the products. support allows some tip flexibility that makes these probes ideal for insertion into small structures. Propionibacterium acnes (P. acnes) are immobilized on inner surface These bacteria produce lipase hydrolyzes model emulsion flowing through transformation vitro as changes caused triglyceride free fatty acids. acids adsorb their carboxylates, and they can be readily identified characteristic spectra (carboxylate marker ~ 1390 cm-1).
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G. Sauermann, A. Herpens, U. Hoppe, A. Kligman, A Novel Fluorimetric Method to Investigate Sebaceous Glands in Humans Springer, Berlin, Heidelberg. pp. 252- 271 ,(1993) , 10.1007/978-3-642-78157-5_17
NANNA Y. SCHURER, PETER M. ELIAS, The biochemistry and function of stratum corneum lipids. Advances in lipid research. ,vol. 24, pp. 27- 56 ,(1991) , 10.1016/B978-0-12-024924-4.50006-7
Albert M. Kligman, Walter B. Shelley, An Investigation of the Biology of the Human Sebaceous Gland11From the Department of Dermatology (Dr. Donald M. Pillsbury, Director), University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania. Journal of Investigative Dermatology. ,vol. 30, pp. 99- 125 ,(1958) , 10.1038/JID.1958.23
Robert E. Kellum, Kathe Strangfeld, Triglyceride Hydrolysis by Corynebacterium Acnes in Vitro**From the Department of Dermatology, University of Oregon Medical School, Portland, Oregon 97201. Journal of Investigative Dermatology. ,vol. 52, pp. 255- 258 ,(1969) , 10.1038/JID.1969.39
Linda M. Lieb, Andrew P. Liimatta, Robert N. Bryan, Bob D. Brown, Gerald G. Krueger, Description of the intrafollicular delivery of large molecular weight molecules to follicles of human scalp skin in vitro Journal of Pharmaceutical Sciences. ,vol. 86, pp. 1022- 1029 ,(1997) , 10.1021/JS9700053
Alan R. Shalita, Genesis of Free Fatty Acids Journal of Investigative Dermatology. ,vol. 62, pp. 332- 335 ,(1974) , 10.1111/1523-1747.EP12724289
J.A. Whiteside, J.G. Voss, Incidence and Lipolytic Activity of Propionibacterium Acnes (Corynebacterium Acnes Group I) And P. Granulosum (C. Acnes Group II) in Acne and in Normal Skin Journal of Investigative Dermatology. ,vol. 60, pp. 94- 97 ,(1973) , 10.1111/1523-1747.EP12724177
Donald T. Downing, John S. Strauss, Lawrence A. Norton, Peter E. Pochi, Mary Ellen. Stewart, THE TIME COURSE OF LIPID FORMATION IN HUMAN SEBACEOUS GLANDS Journal of Investigative Dermatology. ,vol. 69, pp. 407- 412 ,(1977) , 10.1111/1523-1747.EP12510327
Ronald M. Reisner, Donald Z. Silver, Madli Puhvel, Thomas H. Sternberg, Lipolytic Activity of Corynebacterium Acnes**From the Department of Medicine, Division of Dermatology, Harbor General Hospital, Torrance, California 90509, and the Department of Medicine.Division of Dermatology, UCLA School of Medicine, Los Angeles, California 90024. Journal of Investigative Dermatology. ,vol. 51, pp. 190- 196 ,(1968) , 10.1038/JID.1968.113
A. R. Siirila, P. W. Bohn, Kinetics of carboxylic acid surface displacement reactions: a coupled surface plasmon and surface Raman scattering investigation Langmuir. ,vol. 7, pp. 2188- 2195 ,(1991) , 10.1021/LA00058A036