Response elements of the androgen-regulated C3 gene.

摘要: Intron and 5'-flanking regions of the androgen-regulated C3 subunit gene contain potential cis-acting transcription control sequences including several 15-base pair (bp) partial palindromes resembling response elements for glucocorticoid (GRE) progesterone (PRE) receptors. Specific DNA binding androgen receptor (AR) androgen-dependent activation indicate that some these GRE/PRE-like are capable functioning as (ARE). A 0.3-kilobase (kbp) fragment promoter region contains one such sequence (element A) a 0.5-kbp first intron two (elements B C). Androgen-dependent enhancement was assayed by cotransfection CV1 cells with rat AR expression vector, pCMVrAR, genomic fragments or synthetic cloned into reporter vector ptkCAT. Enhancement chloramphenicol acetyltransferase activity 16 +/- 4-fold, while 0.3-kbp no detected element C alone greater than A. Binding in mobility shift assay correlated activity. The intensity transcriptional suggested other regulatory within this potentiated ARE activities C. strongest (C) similar to potent GRE M) mouse mammary tumor virus gene.