Remnants of secondarily necrotic cells fuel inflammation in systemic lupus erythematosus

摘要: Objective Patients with systemic lupus erythematosus (SLE) are often characterized by cellular as well humoral deficiencies in the recognition and phagocytosis of dead dying cells. The aim this study was to investigate whether remnants apoptotic cells involved induction inflammatory cytokines blood-borne phagocytes. Methods We used ex vivo assays comprising components isolated granulocytes monocytes secondarily necrotic cell–derived material (SNEC). Cytokines were measured multiplex bead array technology. Results We confirmed impaired uptake various particulate targets, including immunoglobulin-opsonized beads, from patients SLE compared healthy control subjects. Surprisingly, phagocytes two-thirds took up SNEC, which rarely phagocytosed subjects or rheumatoid arthritis. Supplementation donor blood IgG fractions derived transferred capability take SNEC donors. Phagocytosis-promoting immune globulins also induced secretion huge amounts following SNEC. Conclusion Opsonization autoantibodies fosters its granulocytes. Autoantibody-mediated is accompanied cytokines, fueling inflammation that contributes perpetuation autoimmunity SLE.