Effects of tetracycline on entomopathogenic nematodes and their bacterial symbionts.

摘要: Among the new contaminants relevant for environment, one of most significant roles is played by pharmaceuticals like antibiotic products either human or veterinary use. Their presence could cause serious damage to bacteria and microfauna, nematodes. Within widely investigated nematodes, very little known about interaction between antibiotics entomopathogenic nematodes (EPN). EPNs have been used biological control crops, due their ability penetrate arthropod pests kill hosts thanks a complex symbiotic mechanism with specific gram-negative bacteria. Tetracycline an in medicine, both therapeutic purposes growth livestock. Since its action against documented, we verified this study survival, pathogenicity two species EPNs, Steinernema vulcanicum S. feltiae. All tests were performed tetracycline 1% ethanol solution up 300 mg/L. Apparently, incubation did not harm vitality EPNs. Both as feltiae recovered after 48 h. Moreover, latter EPN grow nor reproduce hemolymph Greater Wax Moth, Galleria mellonella, endosymbionts on MacConkey Agar. Our results suggest that first has always retained all abilities acquired resistance tetracycline, while experiments second provided some contrasting time will require further investigations.