Bence Jones proteins bind to a common peptide segment of Tamm-Horsfall glycoprotein to promote heterotypic aggregation.

摘要: Bence Jones proteins (BJPs) are the major pathogenic factor causing cast nephropathy ("myeloma kidney") by coaggregation with Tamm-Horsfall glycoprotein (THP). Understanding interaction between these is therefore important in developing treatment strategies to prevent renal failure from formation multiple myeloma. We developed an enzyme-linked immunoassay examine this phenomenon. Five different human BJPs (four kappa and one lambda immunoglobulin light chains) were used assay that demonstrated bound THP affinity. competed among themselves for binding THP. The site was a peptide portion of since also deglycosylated Also, monoclonal antibody directed against segment prevented BJPs. By altering conformation THP, reducing agents decreased two concentration-dependent fashion. In turbidity studies, agent, dithiothreitol, coaggregation. Deglycosylated did not coaggregate concluded ionic specific on promoted heterotypic carbohydrate moiety essential coaggregation, perhaps facilitating homotypic aggregation