Lentivirus-Mediated RNA Interference Targeting RhoA Slacks the Migration, Proliferation, and Myelin Formation of Schwann Cells

摘要: RhoA, a member of Rho GTPases family, is known to play an important role in remodeling actin cytoskeleton. During the development peripheral nervous system (PNS), Schwann cells undergo proliferation, migration, and radial sorting finally wrap related axons compactly form myelin sheath. All these processes involve cytoskeletal remodeling. However, RhoA on cell during still unclear. To address this question, we first used lentiviral vector-mediated short hairpin (sh) RNA targeting knock down expression cultured vitro. Effects proliferation migration were examined by BrdU assay transwell assay, respectively. Results present study indicated that downregulated significantly slacked cells' capabilities proliferation. Then, investigated developing rat sciatic nerves. Immunohistology Western blotting showed was mainly expressed nerves peaked at 2 weeks postnatal then kept low level up 8 weeks. In subjected rats whose microinjected with vectors 3 days, found lentiviruses transfected cells, knocked down. Four weeks after lentivirus microinjection, immunohistology transmission electron microscopy illustrated knockdown resulted hypomyelination significant decrease thickness area. Overall data current suggested plays critical biology essential for myelination nerve.