Determination of MK-0767 enantiomers in human plasma by normal phase LC-MS/MS.
作者： K YAN , H SONG , M LO
DOI: 10.1016/J.JCHROMB.2004.09.016
关键词:
摘要: Abstract A sensitive and selective analytical method for the enantioselective determination of MK-0767, a dual peroxisome proliferator-activated receptor (PPAR) α/γ agonist, in human plasma has been developed validated. The chromatography is based on normal-phase chiral separation Kromasil, 5 μm, CHI-DMB 250 mm × 4.6 mm column. detection involves direct introduction normal phase eluent into MS/MS without addition post-column reagent. Atmospheric pressure chemical ionization (APcI) mode was selected as ion source this method. With proper sample handling processing procedures, ex vivo interconversion enantiomers kept to minimum during collection, preparation short term storage frozen samples. successfully utilized determine concentrations MK-0767 support pharmacokinetic investigation man.
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