Protective Effect of Sulforaphane against Dopaminergic Cell Death

摘要: Parkinson's disease (PD) is a progressive neurodegenerative disorder with selective loss of dopaminergic neurons in the substantia nigra. Evidence suggests oxidation dopamine (DA) to DA quinone and consequent oxidative stress as major factor contributing this vulnerability. We have previously observed that exposure or induction NAD(P)H:quinone reductase (QR1), enzyme catalyzes reduction quinone, effectively protects cells. Sulforaphane (SF) drug identified potent inducer QR1 various non-neuronal In present study, we show SF against compounds known induce production (6-hydroxydopamine tetrahydrobiopterin) DAergic cell lines CATH.a SK-N-BE(2)C well mesencephalic neurons. leads attenuation increase protein-bound tetrahydrobiopterin-treated cells, but does not occur cells been depleted DA, suggesting involvement quinone. pretreatment prevents membrane damage, DNA fragmentation, accumulation reactive oxygen species. causes increases mRNA levels enzymatic activity dose-dependent manner. Taken together, these results indicate gene expression, removal intracellular protection toxicity Thus, isothiocyanate found cruciferous vegetables may serve potential candidate for development treatment and/or prevention PD.