Microbial effects on the development of forensically important blow fly species.

摘要: Colonisation times and development rates of specific blow fly species are used to estimate the minimum Post Mortem Interval (mPMI). The presence or absence bacteria on a corpse can potentially affect survival larvae. Therefore an understanding microbial-insect interactions is important for improving interpretation mPMI estimations. In this study, effect two (Escherichia coli Staphylococcus aureus) growth rate three forensically (Lucilia sericata, Calliphora vicina vomitoria) was investigated. Sterile larvae were raised in controlled environment (16:8h day: night light cycle, 23:21°C temperature cycle constant 35% relative humidity) four artificial diets prepared with 100μl 10(5) CFU bacterial solutions as follows: (1) E. coli, (2) S. aureus, (3) 50:50 coli:S. aureus mix (4) sterile bacteria-free control diet. Daily measurements (length, width weight) taken from first instar through emergence adult flies. Survival also determined at pupation emergence. Results indicate that not essential any species. However, larval affected by diet, effects differing between Peak weights varied according species-diet combination; C. vomitoria had largest weight mixed diets, treatment no significant peak L. sericata. These results potential exposed during alter both developmental some