Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq Sequencing

摘要: Metal corrosion is of worldwide concern because it the cause major economic losses, and creates significant safety issues. The mechanism process, as influenced by bacteria, has been studied extensively. However, bacterial communities that create biofilms form on metals are complicated, have not well studied. This why we sought to analyse composition living steel structures, together with influence ecological factors these communities. samples were collected from rust layers plates immersed in seawater for two different periods at Sanya Xiamen, China. We analysed targeted 16S rRNA gene (V3-V4 region) sequencing using Illumina MiSeq. Phylogenetic analysis revealed bacteria fell into 13 phylotypes (similarity level = 97%). Proteobacteria, Firmicutes Bacteroidetes dominant phyla, accounting 88.84% total. Deltaproteobacteria, Clostridia Gammaproteobacteria classes，and accounted 70.90% Desulfovibrio spp., Desulfobacter spp. Desulfotomaculum genera 45.87% These sulphate-reducing known corrode steel. Bacterial diversity six month immersion was much higher than had eight years (P 0.05, Student’s t-test). Overall, data showed carried many species. community time. results our study will be benefit further studies mechanisms resistance.