Use of Mono Q high-resolution ion-exchange chromatography to obtain highly pure and active Escherichia coli RNA polymerase.
作者： Dayle A. Hager , Ding Jun Jin , Richard R. Burgess
DOI: 10.1021/BI00486A016
关键词:
摘要: A method for the purification of highly pure and active Escherichia coli RNA polymerase holoenzyme is described. This simple, reproducible, can be performed at room temperature. The procedure involves high-performance liquid chromatography a partially purified sample on Mono Q ion-exchange column. Under conditions used, well separated from core other impurities. contains virtually no impurities as judged by SDS-polyacrylamide gel electrophoresis. sigma 70 subunit in stoichiometric amounts least 90% active.
acs.org
sci-hub.se 参考文章(20)
D.E. Johnston, W.R. McClure, C.L. Cech, A steady state assay for the RNA polymerase initiation reaction. Journal of Biological Chemistry. ,vol. 253, pp. 8941- 8948 ,(1978) , 10.1016/S0021-9258(17)34268-0
L.R. Yarbrough, J.G. Schlageck, M. Baughman, Synthesis and properties of fluorescent nucleotide substrates for DNA-dependent RNA polymerases. Journal of Biological Chemistry. ,vol. 254, pp. 12069- 12073 ,(1979) , 10.1016/S0021-9258(19)86429-3
D G Somers, M L Pearson, C J Ingles, Isolation and characterization of an alpha-amanitin-resistant rat myoblast mutant cell line possessing alpha-amanitin-resistant RNA polymerase II. Journal of Biological Chemistry. ,vol. 250, pp. 4825- 4831 ,(1975) , 10.1016/S0021-9258(19)41243-X
David Hand, Barry M. Elliott, Martin Griffin, Expression of the cytosolic and particulate forms of transglutaminase during chemically induced rat liver carcinogenesis Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. ,vol. 970, pp. 137- 145 ,(1988) , 10.1016/0167-4889(88)90172-3
Jung-Hye Roe, Richard R. Burgess, M.Thomas Record, Kinetics and mechanism of the interaction of Escherichia coli RNA polymerase with the λPR promoter Journal of Molecular Biology. ,vol. 176, pp. 495- 522 ,(1984) , 10.1016/0022-2836(84)90174-8
Paul Goldsmith, Peter S. Backlund, Kevin Rossiter, Anthony Carter, Graeme Milligan, Cecilia G. Unson, Allen Spiegel, Purification of heterotrimeric GTP-binding proteins from brain: identification of a novel form of Go. Biochemistry. ,vol. 27, pp. 7085- 7090 ,(1988) , 10.1021/BI00418A062
Peter A. Lowe, Dayle A. Hager, Richard R. Burgess, Purification and properties of the sigma subunit of Escherichia coli DNA-dependent RNA polymerase. Biochemistry. ,vol. 18, pp. 1344- 1352 ,(1979) , 10.1021/BI00574A034
S B Mulrooney, M J Lynch, H L Mobley, R P Hausinger, Purification, characterization, and genetic organization of recombinant Providencia stuartii urease expressed by Escherichia coli. Journal of Bacteriology. ,vol. 170, pp. 2202- 2207 ,(1988) , 10.1128/JB.170.5.2202-2207.1988
Jack Greenblatt, Joyce Li, Interaction of the sigma factor and the nusA gene protein of E. coli with RNA polymerase in the initiation-termination cycle of transcription Cell. ,vol. 24, pp. 421- 428 ,(1981) , 10.1016/0092-8674(81)90332-9
E. Bertrand-Burggraf, J.F. Lefèvre, M. Daune, A new experimental approach for studying the association between RNA polymerase and thetetpromoter of pBR322 Nucleic Acids Research. ,vol. 12, pp. 1697- 1706 ,(1984) , 10.1093/NAR/12.3.1697