The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues

摘要: Non-enzymatic modification of proteins by carbohydrates, known as glycation, leads to generation advanced glycation end-products (AGEs). In our study we used in vitro generated AGEs model vivo. We discovered vivo analogs unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized under anhydrous conditions with bovine serum albumin and myoglobin. Using nuclear magnetic resonance spectroscopy have identified a set isomers, open-chain cyclic structures, the fructosamine moiety. mouse anti-MAGE monoclonal antibody show for first time that native previously undescribed analogous product exists living organisms is naturally present tissues both invertebrates vertebrates, including humans. also report MAGE cross-reactive auto-antibodies patients diabetes. anticipate approach modeling will be foundational methodology cell biology. Further studies relevant discovery may contribute clarifying disease mechanisms development novel therapeutic options diabetic complications, neuropathology, cancer.