Recombinant Encephalomyocarditis Viruses Elicit Neutralizing Antibodies against PRRSV and CSFV in Mice.
作者： Shu Zhu , Xin Guo , Lisa R. Keyes , Hanchun Yang , Xinna Ge
DOI: 10.1371/JOURNAL.PONE.0129729
关键词:
摘要: Encephalomyocarditis virus (EMCV) is capable of infecting a wide range species and the infection can cause myocarditis reproductive failure in pigs as well febrile illness human beings. In this study, we introduced entire ORF5 porcine respiratory syndrome (PRRSV) or neutralization epitope regions E2 gene classical swine fever (CSFV), into genome stably attenuated EMCV strain, T1100I. The resultant viable recombinant viruses, CvBJC3m/I-ΔGP5 CvBJC3m/I-E2, respectively expressed partial PRRSV envelope protein GP5 CSFV A1A2 along with proteins. These heterologous proteins fused to N-terminal nonstructural leader could be recognized by anti-GP5 anti-E2 antibody. We also tested immunogenicity these fusion immunizing BALB/c mice viruses. immunized animals elicited neutralizing antibodies against CSFV. Our results suggest that engineered an expression vector serve tool development novel live vaccines various animal species.
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