The effects of cardamonin on lipopolysaccharide-induced inflammatory protein production and MAP kinase and NFkappaB signalling pathways in monocytes/macrophages.

摘要: Background and purpose: In this study we examined the effect of natural product cardamonin, upon lipopolysaccharide (LPS)-induced inflammatory gene expression in order to attempt pinpoint mechanism action. Experimental approaches: Cardamonin was isolated from Greek plant A. absinthium L. Its effects were assessed on LPSinduced nitrite release iNOS COX-2 protein two macrophage cell lines. Western blotting used investigate its phosphorylation mitogen activated (MAP) kinases, ERK, JNK p38 MAP kinase, activation NFkB pathway, at level IkBa degradation NFkB. Also GAS/GAF-DNA binding by EMSA. Key results: concentration-dependently inhibited both NO but had no expression. It did not affect or However, it DNA-binding LPS-stimulated cells nuclear extracts (in vitro). also IFNg-stimulated induction binding. Conclusions implications: These results show that inhibitory cardamonin LPS-induced is mediated via initial kinase pathways due a direct transcription factor DNA. although some selectivity cardamonin’s action implicated inability expression, exact mechanism(s) has yet be identified.