ISOLATION AND SEQUENCE OF CDNA ENCODING A 3-METHYLCHOLANTHRENE-INDUCIBLE CYTOCHROME P450 FROM WILD RED SEA BREAM, PAGRUS MAJOR

摘要: We have isolated a cDNA clone of mRNA for the cytochrome P450 from 3-methylcholanthrene (MC)-treated red sea bream,Pagrus major, using fragment rat P4501A2 as probe. The cloned is ca. 1.8 kb long and contains an open reading frame 1545 nucleotides polypeptides 515 amino acids. deduced N-terminal acid sequence very similar to that purified protein marine fish scup, which was reported previously (Klotz et al. 1983). A conserved containing putative heme-binding cysteine present in equivalent position proximate C-terminus molecules. shows more than 50% positional identity with known members mammalian aromatic hydrocarbon-inducible family. RNA blot analysis indicates (s) expressed liver, kidney, gill gut MC-treatedP. major.