Yeast cells expressing modified G proteins and methods of use therefor
作者： Joshua Trueheart , Dana M. Fowlkes , Andrew J. M. Murphy , James R. Broach , Jeremy I. Paul
DOI:
关键词:
摘要: The present invention pertains to novel yeast cells which are useful for the expression of heterologous G protein coupled receptors. can be used in screening assays screen modulators Specifically, provides express a receptor and mutant and/or chimeric subunit molecules serve functionally integrate into pheromone signaling pathway cell. also receptors integrated cell membrane using α factor leader sequence. Drug discovery subject provided.
lens.org 参考文章(110)
M Russell, G L Johnson, G protein amino-terminal alpha i2/alpha s chimeras reveal amino acids important in regulating alpha s activity. Molecular Pharmacology. ,vol. 44, pp. 255- 263 ,(1993)
Erickson D, Intercepted messages. New biotechnology drugs target intracellular communication. Scientific American. ,vol. 267, pp. 122- 123 ,(1992)
Wright Na, Alison Mr, Growth factors and growth factor receptors. British Journal of Hospital Medicine. ,vol. 49, pp. 774- 774 ,(1993)
Bates S, Peters G, Hall M, Evidence for different modes of action of cyclin-dependent kinase inhibitors: p15 and p16 bind to kinases, p21 and p27 bind to cyclins. Oncogene. ,vol. 11, pp. 1581- 1588 ,(1995)
R.E. Sterne, J. Thorner, K. Kuchler, Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells. The EMBO Journal. ,vol. 8, pp. 3973- 3984 ,(1989) , 10.1002/J.1460-2075.1989.TB08580.X
E. Leberer, D. Dignard, L. Hougan, D.Y. Thomas, M. Whiteway, Dominant-negative mutants of a yeast G-protein beta subunit identify two functional regions involved in pheromone signalling. The EMBO Journal. ,vol. 11, pp. 4805- 4813 ,(1992) , 10.1002/J.1460-2075.1992.TB05586.X
Steve Arkinstall, S. P. Watson, The G-Protein Linked Receptor Facts Book ,(1994)
Arthur E. Franke, Glenn C. Andrews, Norma P. Stimler-Gerard, Craig J. Gerard, Henry J. Showell, [49] Human C5a anaphylatoxin: Gene synthesis, expression, and recovery of biologically active material from Escherichia coli Methods in Enzymology. ,vol. 162, pp. 653- 668 ,(1988) , 10.1016/0076-6879(88)62107-0
Herrmann F, Brach Ma, Belka C, The role of tyrosine kinases and their substrates in signal transmission of hematopoietic growth factors: a short review. Leukemia. ,vol. 9, pp. 754- 761 ,(1995)
Bruce R. Conklin, Zvi Farfel, Kevin D. Lustig, David Julius, Henry R. Bourne, Substitution of three amino acids switches receptor specificity of Gqα to that of Giα Nature. ,vol. 363, pp. 274- 276 ,(1993) , 10.1038/363274A0